US prosecutors will soon call for the extradition of Abu Agila Mohammad to stand trial in the United States, according to US media.
The bombing spurred global investigations and produced sanctions against Libya, which ultimately surrendered intelligence officials wanted in the attacks for prosecution in Europe.
The announcement of new prosecution would likely carry personal significance for Attorney General William Barr, who leaves the position next week.
He held the same job when the Justice Department revealed criminal charges nearly 30 years ago against the intelligence officials.
News of the criminal case was first reported by The Wall Street Journal and The New York Times.
A person familiar with the Justice Department's plan who was not authorised to discuss it by name confirmed it on condition of anonymity.
The New York-bound flight exploded over Lockerbie less than an hour after take-off from London on December 21, 1988.
Among the Americans on board were 35 Syracuse University students flying home for Christmas after a semester abroad.
The attack, caused by a bomb packed into a suitcase, killed 259 people on the plane and 11 on the ground.
One man - former Libyan intelligence official Abdel Baset al-Megrahi - was convicted of the bombing.
A second Libyan suspect was acquitted of all charges.
Al-Megrahi was given a life sentence, but Scottish authorities released him on humanitarian grounds in 2009 when he was diagnosed with prostate cancer.
He later died in Tripoli.
